Calmodulin activity in yeast and mycelial phases of Ceratocystis ulmi |
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Authors: | Ganapathy Muthukumar Maureen T Luby Kenneth W Nickerson |
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Institution: | School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0118, U.S.A. |
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Abstract: | Abstract The yeast and mycelial phases of Ceratocystis ulmi contained roughly equivalent levels of calmodulin activity as determined by their ability to stimulate calmodulin-deficient bovine brain cAMP phosphodiesterase. This stimulation was calcium-dependent and could be inhibited by either dibucaine or trifluoperazine. Also, the concentration of dibucaine necessary to achieve the mycelium-to-yeast morphological conversion was found to be 3-fold greater in the presence of exogenous calcium. A model is presented in which only 30% of the cellular calmodulin need be complexed with calcium ions for mycelial development. |
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Keywords: | Ceratocystis ulmi calmodulin dimorphism calcium trifluoperazine cyclic nucleotide phosphodiesterase |
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