Effect of sugar chain structure on in vivo biological activity of a recombinant human glycoprotein expressed in SP2/0-AG14 cells |
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| Authors: | Shinji Sugaya Hideya Ohashi Takao Shinozawa |
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| Affiliation: | (1) Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University Kiryu, Gunma, 376, Japan |
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| Abstract: | We have reported previously that recombinant human erythropoietin (rhEPO) produced from Sp2/0-Ag14 transformant had a low biological activity in vivo as compared with other human EPOs. rhEPO from SP2/0 has now been found to have a lower amount of sialic acid and different sugar chains from other hEPOs. This recombinant molecule contains disialobranches as major and complex sugar chains which showed broad peaks on gel-filtration chromatography. These differences may be responsible for its low in vivo bioactivity. |
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