Screening and degradation performances of dominant strains in high-salinity landfill leachate |
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Authors: | Li Guo Min Ji Huiyu Dong Yanjie Wei |
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Affiliation: | (1) School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China;(2) Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Department of Fishery Sciences, Tianjin Agricultural University, Tianjin, 300384, China |
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Abstract: | In order to enhance the removal efficiency of chemical oxygen demand (COD) in the high-salinity landfill leachate, the dominant strains were isolated from high-salinity landfill leachate. The dominant strains and bacteria consortium were screened for COD treatment potential using an aerobic COD concentration decrease test. Ten strains, TJ01–TJ10, were isolated, of which six strains TJ02, TJ03, TJ05, TJ06, TJ07, and TJ09 were found to have higher COD removal when the single bacteria were added, all more than 20%. The most effective combination was TJ06 + TJ09; the COD removal efficiency reached 45.57%. 16S rDNA gene sequence analysis revealed that TJ06 and TJ09 belonged to the genus Bacillus. The effects of the dominant bacteria consortium on the high-salinity landfill leachate varied with pH value and the volume fraction of leachate. The COD removal efficiencies maintained higher when the pH value was 6–8 and the volume fractions of leachate were less than 80%. The result also suggested that there is little effect on the growth of TJ06 and TJ09 when the range of Cl− concentration is 0–30,000 mg/L. |
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Keywords: | Landfill leachate Dominant strains Salinity Chemical oxygen demand (COD) |
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