绿盲蝽腹神经节的解剖结构

【目的】揭示绿盲蝽Apolygus lucorum腹神经节的组成结构。【方法】采用免疫组织化学染色方法，利用突触蛋白抗体对绿盲蝽成虫的腹神经节进行免疫标记，激光共聚焦扫描显微镜扫描照相获得原始数据，用图像分析软件进行标记，构建三维结构模型。【结果】绿盲蝽成虫腹神经节位于腹神经索的末端，与其前方的后胸神经节和中胸神经节紧密融合，形成后部神经节。与脑和胸神经节类似，腹神经节由周围的细胞体和内部的神经髓构成。腹神经节的神经纤维束主要包括位于腹侧的两条纵向神经连索和向两侧发出的9束神经纤维。9束神经纤维连接着9个神经原节，即富含突触联系的神经髓。这些神经原节紧密融合，无明显的边界，最后两节形成膨大的末端腹神经节。两侧的神经原节由横向的神经连锁连接起来。腹神经节外周的细胞体数量较多，排列紧密，大小一致，仅在前端背侧中间和后端腹侧中间位置分别有2个和5个体积较大的细胞体。【结论】本研究结果明确了绿盲蝽腹神经节的结构，为进一步研究昆虫的行为调控及神经系统发育和演化奠定一定的形态学基础。

Anatomical structure of the abdominal ganglion of Apolygus lucorum (Hemiptera: Miridae)

【Aim】 To reveal the anatomical organization of the abdominal ganglion of the green mirid bug, Apolygus lucorum (Hemiptera: Miridae). 【Methods】 The neuropils of the abdominal ganglion of A. lucorum adults were labeled by immunohistochemical staining with synaptic protein antibody. Digital images of the abdominal ganglion were obtained by using a confocal laser scanning microscope. The three-dimensional models were reconstructed by using image analysis software. 【Results】 The abdominal ganglion of A. lucorum adults is located at the distal end of the ventral nerve cord, fused with the mesothoracic and metathoracic ganglia and becoming a posterior ganglion. Like brain and thoracic ganglia, the abdominal ganglion is composed of outer somata and inner neuropils. Nerve fibers of the abdominal ganglion include two connectives in ventral and nine pairs of nerve bundles given off from the lateral. Nine nerve bundles are respectively connected with nine neuromeres full of synapsin. These neuromeres are fused tightly without distinct boundary. The last two neuromeres are expanded forming the terminal abdominal ganglion (TAG). Bilateral neuromeres are connected by transverse commissures. Around the abdominal ganglion, there are massive, small and close packed somata. In the middle of the anterior dorsal portion and the posterior ventral portion, there are two and five big somata, respectively. 【Conclusion】 The results demonstrate the anatomical organization of the abdominal ganglion of A. lucorum and provide the morphological basis for the study of behavioral regulation and the development and evolution of nervous system of insects.