| 环糊精葡萄糖基转移酶高效异源表达研究进展 |
| |
| 引用本文： | 李晓涵,郝建华,郭姣梅,王伟,孙晶晶,刘均忠.环糊精葡萄糖基转移酶高效异源表达研究进展[J].微生物学通报,2020,47(2):615-622. |
| |
| 作者姓名： | 李晓涵 郝建华 郭姣梅 王伟 孙晶晶 刘均忠 |
| |
| 作者单位： | 2 上海海洋大学食品学院 上海 201306,1 农业农村部极地渔业开发重点实验室 青岛海洋科学与技术试点国家实验室 海洋药物与生物制品功能实验室 中国水产科学研究院黄海水产研究所 山东 青岛 266071;3 江苏省海洋生物产业技术协同创新中心 江苏 连云港 222005,2 上海海洋大学食品学院 上海 201306,1 农业农村部极地渔业开发重点实验室 青岛海洋科学与技术试点国家实验室 海洋药物与生物制品功能实验室 中国水产科学研究院黄海水产研究所 山东 青岛 266071,1 农业农村部极地渔业开发重点实验室 青岛海洋科学与技术试点国家实验室 海洋药物与生物制品功能实验室 中国水产科学研究院黄海水产研究所 山东 青岛 266071,1 农业农村部极地渔业开发重点实验室 青岛海洋科学与技术试点国家实验室 海洋药物与生物制品功能实验室 中国水产科学研究院黄海水产研究所 山东 青岛 266071 |
| |
| 基金项目： | 国家重点研发计划(2018YFC0311106)；青岛市市南区科技发展计划(2018-4-002-ZH) |
| |
| 摘 要： | 环糊精葡萄糖基转移酶(cyclodextringlycosyltransferase,CGTase)酶法合成环糊精是目前生产环糊精的主要方法。本文介绍了用于生产环糊精葡萄糖基转移酶的几种工程菌株:大肠杆菌、枯草芽孢杆菌以及毕赤酵母,其中大肠杆菌是目前应用最广泛的用于表达CGTase的表达系统。除此之外,本文还总结了高效表达环糊精葡萄糖基转移酶的有效策略:选择合适的表达载体、启动子以及信号肽,以及密码子优化和分子伴侣共表达,以期为在相关CGTase研究领域开展研究提供参考。
|
| 关 键 词： | 环糊精葡萄糖基转移酶，大肠杆菌，枯草芽孢杆菌，酵母，异源表达，策略 |
Advance in high-level heterologous expression of cyclodextrin glycosyltransferase |
| |
| LI Xiao-Han,HAO Jian-Hu,GUO Jiao-Mei,WANG Wei,SUN Jing-Jing and LIU Jun-Zhong.Advance in high-level heterologous expression of cyclodextrin glycosyltransferase[J].Microbiology,2020,47(2):615-622. |
| |
| Authors: | LI Xiao-Han HAO Jian-Hu GUO Jiao-Mei WANG Wei SUN Jing-Jing and LIU Jun-Zhong |
| |
| Institution: | 2 College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China,1 Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Drugs and Bioproducts, Yellow Sea Fisheries Research Institute, Qingdao, Shangdong 266071, China;3 Jiangsu Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resource, Lianyungang, Jiangsu 222005, China,2 College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China,1 Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Drugs and Bioproducts, Yellow Sea Fisheries Research Institute, Qingdao, Shangdong 266071, China,1 Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Drugs and Bioproducts, Yellow Sea Fisheries Research Institute, Qingdao, Shangdong 266071, China and 1 Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Drugs and Bioproducts, Yellow Sea Fisheries Research Institute, Qingdao, Shangdong 266071, China |
| |
| Abstract: | The cyclodextrin glycosyltransferases (CGTases) are enzymes that used to synthesize cyclodextrin, and this method of production of cyclodextrin is mostly used at present. we described several engineering strains which were used to produce CGTase including Escherichia coli, Bacillus subtilis and Pichia pastoris, and the expression system of E. coli is one of the most widely used. In addition, we also summarized the effective strategies for high efficient expression of CGTase including appropriate expression vector, promoter and signal peptide, codon optimization, co-expression with molecular chaperone. The purpose of this review is to provide a reference for relevant research of CGTase. |
| |
| Keywords: | Cyclodextrin glycosyltransferase (CGTase) Escherichia coli Bacillus subtilis Yeast Heterologous expression Strategy |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
