不同品种苹果树内生细菌群落多样性及功能

【背景】目前苹果树内生细菌的研究较多,但对不同品种苹果树内生细菌群落多样性分析比较的相关报道还较少。【目的】通过分析比较新疆本地和吉尔吉斯斯坦引进的8个不同品种苹果树内生细菌群落多样性的差异,可以充分挖掘其蕴含的丰富微生物资源。【方法】采用MiSeq高通量测序技术分别测定不同品种苹果树内生细菌群落16S rRNA基因V3-V4区序列并进行生物信息学分析。【结果】不同品种苹果树中获得的V3-V4区有效序列数在61487-71583条之间,聚成24-92个操作分类单元(operationaltaxonomicunit,OTU),Shannon指数和Simpson指数分别在0.729-1.177和0.265-0.457之间,新疆本地品种的苹果树内生细菌种类和多样性高于吉尔吉斯斯坦品种。内生细菌种群分析结果表明,变形菌门和放线菌门的OTU总计分别覆盖了不同品种苹果树内生细菌的61.16%-97.08%,为苹果树的主要优势细菌门。优势细菌属数目、组成及其丰度随苹果品种的不同而有所差异。马赛菌属(Massilia)和节杆菌属(Arthrobacter)的总丰度最高,其丰度分别在6.06%-71.3...

Endophytic bacterial community diversity and function in apple trees of different varieties

Background] It was rare to compare the difference of endophytic bacterial communities in apple varieties thought many investigations had been reported on endophytic bacteria in apple trees recently. Objective] By analyzing and comparing the diversity of endophytic bacterial communities in apple trees of 8 different varieties in Xinjiang and Kyrgyzstan, the abundant microbial resources would be explored. Methods] Using MiSeq high-throughput sequencing method, sequences at V3?V4 regions of the 16S rRNA gene were amplified, then communities and functions of endophytic bacteria were analyzed in these apple varieties. Results] valid sequences of 61 487?71 583 bp were obtained from varieties of apple trees, respectively, which clustered into 24?92 distinct operational taxonomic units (OTUs). Shannon index and Simpson index were 0.729?1.177 and 0.265?0.457, respectively. Species diversities of endophytic bacteria in Xinjiang apple varieties were richer than that in Kyrgyzstan varieties. The taxonomic analysis of endophytic bacteria showed that total OTUs of Proteobacteria and Actinobacteria covered 61.16%?97.08% of endophytic bacteria in different apple trees, respectively. They were the dominant bacteria phylum of apple trees. The number, composition and abundance of dominant genus were different in these varieties. OTUs in the genus Massilia and Arthrobacter were the richest, ranging from 6.06%?71.37% and 1.29%?17.86%, respectively. There were some microbial groups of beneficial functional characteristics related to promoting growth, stress resistance or degradation of toxic and harmful substances in the environment in these dominant bacteria. The function prediction showed that the functions of endophytic bacterial community in different apple varieties were different. The function informations of microbial community of Xinjiang varieties were more than that of Kyrgyzstan varieties, which included chemoheterotrophy, aerobic chemoheterotrophy, ureolysis, arsenate detoxification and dissimilatory arsenate reduction. In addition, a large number of unclassified genera were observed in these varieties, ranging from 13.74% to 69.60%. Conclusion] The diversity of endophytic bacterial communities of these apple varieties were relatively rich, and there were significant differences in the composition and function. Meanwhile, it provided clues to mine new microbial resources and explore the function of endophytic bacterial communities in apple varieties.