The Bacillus subtilis menCD promoter is responsive to extracellular pH |
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Authors: | Kevin F. Hill John P. Mueller Harry W. Taber |
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Affiliation: | (1) Department of Microbiology and Immunology, Albany Medical College, 12208 Albany, NY, USA;(2) Present address: Department of Pediatrics, Bethesda Naval Hospital, 20814 Bethesda, MD, USA;(3) Present address: Department of Microbiology and Molecular Biology, Tufts University Health Sciences Campus, 02111 Boston, MA, USA |
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Abstract: | Activation kinetics of a Bacillus subtilis menaquinone biosynthetic gene promoter (the menCD promoter) were measured during growth and sporulation, with the aid of a menCD-lacZ translational gene fusion. Transient maximal activation was seen shortly after the end of exponential growth in unbuffered complex medium containing a low glucose concentration. These activation kinetics were correlated with transient acidification of the medium under conditions permitting TCA cycle function during the post-exponential period, while mutations that blocked TCA cycle function (cit mutants) were associated with sustained acidification and promoter activation during this period. In cit+ strains, buffering of the medium to pH 5.7 caused sustained maximal activation, while buffering to pH 7.2 prevented enhancement of activation. The menCD promoter appears to be responsive to extracellular acidic pH. |
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Keywords: | Menaquinone men genes Bacillus subtilis Promoter pH |
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