Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae |
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| Authors: | Bruno Chevallier Dominique Dugourd Kazimirez Tarasiuk Josée Harel Marcelo Gottschalk Marylène Kobisch Joachim Frey |
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| Institution: | CNEVA-Ploufragan, Les Croix, P.O. Box 53, F-22440 Ploufragan, France;Groupe de Recherche sur les Maladies Infectieuses du Porc, Facultéde Médecine Vétérinaire, Universitéde Montréal, Saint-Hyacinthe, Qué., Canada;National Veterinary Research Institute, 24-100 Pulawy, Poland;Institute for Veterinary Bacteriology, University of Berne, Laenggasstrasse 122, CH-3012 Berne, Switzerland |
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| Abstract: | The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404±40 kb. The chromosome sizes for the reference strains of the other serotypes range between 2.3 and 2.4 Mb. The restriction pattern profiles of AscI, ApaI and NheI digested chromosomes showed a high degree of polymorphism among the different serotype reference strains and allowed their discrimination. The analysis of the macrorestriction pattern polymorphism revealed phylogenetic relationships between the different serotype reference strains which reflect to some extent groups of serotypes known to cross-react serologically. In addition, different pulsed fields gel electrophoresis patterns also revealed heterogeneity in the chromosomal structure among different field strains of serotypes 1, 5a, and 5b, while strains of serotype 9 originating from most distant geographical places showed homogeneous ApaI patterns in pulsed field gel electrophoresis. |
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| Keywords: | Actinobacillus pleuropneumoniae Chromosome size Pulsed field gel electrophoresis |
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