Electrogenicity at the donor/acceptor sides of cyanobacterial photosystem I |
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Authors: | M. D. Mamedov R. M. Gadzhieva K. N. Gourovskaya L. A. Drachev A. Yu. Semenov |
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Affiliation: | (1) A. N. Belozersky Institute of Physico-chemical Biology, Moscow State University, 119899 Moscow, Russia;(2) Academy of Science of Azerbaijan, Institute of Botany, Patamdartskoe shosse 40, 370073 Baku, Azerbaijan Republic |
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Abstract: | To study electrogenesis the photosystem I particles fromSynechococcus elongatus were incorporated into asolectin liposomes, and fast kinetics of laser flash-induced electric potential difference generation has been measured by a direct electrometric method in proteoliposomes adsorbed on a phospholipid-impregnated collodion film. The photoelectric response has been found to involve three electrogenic stages associated with (i) iron-sulfur center Fx reduction by the primary electron donor P700, (ii) electron transfer between iron-sulfur centers Fx and FA/FB, and (iii) reduction of photo-oxidized P700+ by reduced cytochromec553. The relative magnitudes of phases (ii) and (iii) comprised about 20% of phase (i). |
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Keywords: | Photosystem I proteoliposomes electrogenicity electron transfer cytochromec553 iron-sulfur centers Synechococcus elongatus |
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