| 基因芯片技术检测肾综合征出血热病毒核酸的研究 |
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| 引用本文： | 朱进,陶开华,操敏,张云,李越希,张锦海,唐家琪.基因芯片技术检测肾综合征出血热病毒核酸的研究[J].Virologica Sinica,2004,19(1):79-81. |
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| 作者姓名： | 朱进 陶开华 操敏 张云 李越希 张锦海 唐家琪 |
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| 作者单位： | 南京军区军事医学研究所 江苏南京210002
(朱进，陶开华，操敏，张云，李越希，张锦海)，南京军区军事医学研究所 江苏南京210002(唐家琪) |
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| 基金项目： | 全军十五重点课题(01L006) |
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| 摘 要： | To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques, specific primers and probes were designed according to the conservative and specific DNA sequence of 76-118 strain and R22 strain. The probes were spotted on glass slides to form microarrays.The Cy3-1abled single stranded DNA fragments prepared by dissymmetical PCR were hybridized with the probes on the glass slides. The microarrays were scanned and analyzed with a scanner. The results showed that the DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity. The developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and could be widely applied in specific pathogens detection ofinfectious diseases such as hemorrhagic fever with renal syndrome.
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| 关 键 词： | 肾综合征出血热 汉坦病毒 基因芯片 核酸 |
Establishment of Diagnostic Assay for Hantavirus with Microarray Techniques |
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| ZHU Jin,TAO Kai-hua,CAO Min,ZHANG Yun,LI Yue-xi,HANG Jing-hai,TANG Jia-qi.Establishment of Diagnostic Assay for Hantavirus with Microarray Techniques[J].中国病毒学(英文版),2004,19(1):79-81. |
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| Authors: | ZHU Jin TAO Kai-hua CAO Min ZHANG Yun LI Yue-xi HANG Jing-hai TANG Jia-qi |
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| Institution: | ZHU Jin,TAO Kai-hua,CAO Min,ZHANG Yun,LI Yue-xi**,HANG Jing-hai,TANG Jia-qi |
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| Abstract: | To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques, specific primers and probes were designed according to the conservative and specific DNA sequence of 76-118 strain and R22 strain. The probes were spotted on glass slides to form microarrays. The Cy3-labled single stranded DNA fragments prepared by dissymmetical PCR were hybridized with the probes on the glass slides. The microarrays were scanned and analyzed with a scanner. The results showed that the DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity. The developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and could be widely applied in specific pathogens detection of infectious diseases such as hemorrhagic fever with renal syndrome. |
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| Keywords: | Hemorrhagic fever with renal syndrome Hantavirus DNA microarray |
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