Advances in microfluidic PCR for point-of-care infectious disease diagnostics |
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| Authors: | Park Seungkyung Zhang Yi Lin Shin Wang Tza-Huei Yang Samuel |
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| Institution: | aDepartment of Emergency Medicine, Johns Hopkins University, Baltimore, MD 21205, USA;bDepartment of Biomedical Engineering and the Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21218, USA;cDepartment of Medicine, Stanford University, Stanford, CA 94305, USA;dDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA |
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| Abstract: | Global burdens from existing or emerging infectious diseases emphasize the need for point-of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular approaches based on PCR methods have made significant inroads by improving detection time and accuracy but are still largely hampered by resource-intensive processing in centralized laboratories, thereby precluding their routine bedside- or field-use. Microfluidic technologies have enabled miniaturization of PCR processes onto a chip device with potential benefits including speed, cost, portability, throughput, and automation. In this review, we provide an overview of recent advances in microfluidic PCR technologies and discuss practical issues and perspectives related to implementing them into infectious disease diagnostics. |
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| Keywords: | PCR Microfluidic Diagnostic methods Infectious disease Point-of-care |
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