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| 表达鸡传染性支气管炎病毒JS/95/03株S1蛋白的重组杆状病毒构建 | |
| 引用本文： | 戴亚斌,陈德胜,丁铲,潘杰彦,刘兴友,芦银华,刘梅,陈溥言,蔡宝祥.表达鸡传染性支气管炎病毒JS/95/03株S1蛋白的重组杆状病毒构建[J].病毒学报,2003,19(1):86-90. |
| 作者姓名： | 戴亚斌 陈德胜 丁铲 潘杰彦 刘兴友 芦银华 刘梅 陈溥言 蔡宝祥 |
| 作者单位： | 1. 农业部动物疫病诊断和免疫重点实验室,南京农业大学,江苏,南京,210095;江苏省家禽科学研究所,江苏,扬州,225003 2. 农业部动物疫病诊断和免疫重点实验室,南京农业大学,江苏,南京,210095 3. 江苏省家禽科学研究所,江苏,扬州,225003 |
| 基金项目： | 国家自然科学基金;3989329; |
| 摘 要： | The recombinant baculovirus expressing S1 glycoprotein of nephropathogenic strain JS/95/03 of infectious bronchitis virus was generated by using the Bac-to-Bac baculovirus expression system.The BamH I-Sal Ⅰ fragment containing S1 gene from the recombinant plasmid pMDJS9503S1 was purified and cloned in frame into the baculovirus transposing vector pFASTBAC HTa under the polyhedrin gene promoter.The recombinant transposing plasmid pFASTJS9503S1 was screened and then transformed into Escherichia coli DH10BAC.The resulting recombinant bacmid rBacmidJS9503S1 was transfected into cells of the insect Spodoptera frugiperda(Sf9)and the recombinant baculoviruse rAcJS9503S1 was obtained.The lysates of cells infected with rAcJS9503S1 were analyzed by SDS-PAGE and the expressed product of S1 gene was detected by Western bloting and immunofluorescence assay(IFA).The results showed the recombinant baculovirus was fully capable of expressing S1 glycoprotein of JS/95/03.Maybe owing to the incomplete glycosylation in insect cells,the S1 gene product had a Mr of only 61000.In immunofluorescence test and Western blotting,the expressed product could react with polycolonal antibody against IBV M41 strain,indicating it possessed the antigenic properties specific for native S1 glycoprotein. |
| 关 键 词： | 传染性支气管炎病毒（IBV） S1基因 S1蛋白 杆状病毒表达载体 表达 |
| 文章编号： | 1000-8721(2003)01-0086-05 |
Construction of Recombinant Baculovirus Expressing S1 Glycoprotein of Infectious Bronchitis Virus Isolate JS/95/03 |
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| DAI Ya-bin ,CHEN De-sheng ,DING Chan ,PAN Jie-yan ,LIU Xing-you ,LU Yin-hua ,LIU Mei ,CHEN Pu-yan ,CAI Bao-xiang.Construction of Recombinant Baculovirus Expressing S1 Glycoprotein of Infectious Bronchitis Virus Isolate JS/95/03[J].Chinese Journal of Virology,2003,19(1):86-90. | |
| Authors: | DAI Ya-bin CHEN De-sheng DING Chan PAN Jie-yan LIU Xing-you LU Yin-hua LIU Mei CHEN Pu-yan CAI Bao-xiang |
| Institution: | DAI Ya-bin 1,2,CHEN De-sheng 1,DING Chan 2,PAN Jie-yan 1,LIU Xing-you 1,LU Yin-hua 1,LIU Mei 2,CHEN Pu-yan 1,CAI Bao-xiang 1 |
| Abstract: | |
| Keywords: | infectious bronchitis virus(IBV) S1 gene S1 glycoprotein baculovirus expression vector expression |
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