Effect of alimentary thiamine deficiency on the activity of gluconeogenic key enzymes in rat liver and kidney |
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Authors: | A A Maslovskaya N K Lukashik |
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Institution: | Chair of Biochemistry, Medical Institute, Grodno, B. S. S. R., U. S. S. R. |
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Abstract: | Activity of the key enzymes of gluconeogenesis under alimentary thiamine deficiency (15 days of dietary treatment) was studied in the liver and kidney of fed and 48 h starved rats. As compared to pair-fed controls vitamin B1-deficiency was followed by a decrease of glucose 6-phosphatase and fructose 1,6-bisphosphatase activities in both organs; the activity of phosphoenolpyruvate carboxykinase was diminished only in the liver. Starvation of thiamine-deficient rats (as compared to pair-fed starved group) resulted in lower activation of these enzymes. The decrease of the enzyme activities in thiamine-deficient animals indicates that de novo glucose synthesis in the tissues is depressed, though thiamine-requiring enzymes are not directly involved in this process. Possible mechanisms of alterations described are discussed. |
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