Titin mutations as the molecular basis for dilated cardiomyopathy |
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Authors: | Itoh-Satoh Manatsu Hayashi Takeharu Nishi Hirofumi Koga Yoshinori Arimura Takuro Koyanagi Takeshi Takahashi Megumi Hohda Shigeru Ueda Kazuo Nouchi Tatsuhito Hiroe Michiaki Marumo Fumiaki Imaizumi Tsutomu Yasunami Michio Kimura Akinori |
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Affiliation: | Department of Molecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo 101-0062, Japan. |
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Abstract: | Dilated cardiomyopathy (DCM) is a heterogeneous cardiac disease characterized by ventricular dilatation and systolic dysfunction. Recent genetic studies have revealed that mutations in genes for cardiac sarcomere components lead to DCM. The cardiac sarcomere consists of thick and thin filaments and a giant protein, titin. Because one of the loci of familial DCM was mapped to the region of the titin gene, we searched for titin mutations in the patients and identified four possible disease-associated mutations. Two mutations, Val54Met and Ala743Val, were found in the Z-line region of titin and decreased binding affinities of titin to Z-line proteins T-cap/telethonin and alpha-actinin, respectively, in yeast two-hybrid assays. The other two mutations were found in the cardiac-specific N2-B region of titin and one of them was a nonsense mutation, Glu4053ter, presumably encoding for a truncated nonfunctional molecule. These observations suggest that titin mutations may cause DCM in a subset of the patients. |
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Keywords: | dilated cardiomyopathy titin mutation sarcomere Z-line T-cap/telethonin actinin |
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