Pregnancy diagnosis in the domestic horse through direct urinary estrone conjugate analysis |
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Authors: | KL Evans LH Kasman JP Hughes M Couto BL Lasley |
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Institution: | 1. Research Department, Zoological Society of San Diego, San Diego, CA 92112 USA;2. Department of Reproduction, University of California, School of Veterinary Medicine, Davis, CA 95616 USA |
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Abstract: | Urinary estrone conjugates were measured directly by radioimmunoassay (RIA) in 20 pregnancies from preconception diestrus to day 78 of pregnancy. High performance liquid chromatography separation defined estrone sulfate (ES) as the predominant immunoreactive peak which accounted for 94% to 97% of the total immunoreactivity after chromatography. Diestrous values indexed by creatinine were 0.15 ± 0.07 micrograms/mg Cr, as compared to estrous values which rose to 0.47 ± 0.14 micrograms/mg Cr, . Urinary ES concentrations significantly increased (P = 0.0001 in pregnant mares from day 35 to day 47 (1.21 ± 0.12 micrograms/mg Cr) as compared to day 25 to day 34 (0.27 ± 0.01 micrograms/mg Cr). Measurement of urinary ES may provide an alternate or augmentive method of pregnancy diagnosis in the domestic mare. |
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Keywords: | Pregnancy detection urinary estrogens horse |
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