Association in organ culture of the hypophysis and glandular tissue of the testis of Gobius niger L.]

Pituitaries of Gobium niger are previously cultured separately for periods of time varying from 6 to 20 days. Therafter, their gonadotropic potency is tested by associating them during three days with explants of glandular tissue from testis as effectors: in each case, a new fish gives a controll pituitary and three testicular explants. The latter are distributed into (1) controll explant, (2) explant associated with controll pituitary, (3) explant associated with a previously cultured pituitary. The results indicate that a gonadotropic potency subsists in the pituitary even after 20 days of isolated culture.