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THE METABOLISM OF TRITIATED DOPAMINE IN REGIONS OF THE RAT BRAIN IN VIVO—I
Authors:K. M. Taylor   R. Laverty
Affiliation:Wellcome Medical Research Institute, Department of Medicine University of Otago Medical School, Dunedin, New Zealand
Abstract:Abstract— A simple combination of acid- and base-exchange resin columns enabled a more complete separation of amine, acid and neutral metabolites of catecholamines from individual small brain samples to be made. Fractions containing individual catecholamines or metabolites were obtained in aqueous eluates suitable for fluorimetric or radioisotopic analysis. With consistent intraventricular injection and brain dissection techniques, this separation method enables a study of the metabolism of catecholamines in regions of the rat brain and the effects of drugs on this metabolism.
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