|
|||||
|
|
Typing of Staphylococcus aureus by amplification of the 16S–23S rRNA intergenic spacer sequences |
|
| Authors: | Lucilla Dolzani Enrico Tonin Cristina Lagatolla Carlo Monti-Bragadin |
| Institution: | Dipertimento di Biologia Molecolare, Sezione di Microbiologia, Universitàdi Siena, Via Laterina, 8, 53100 Siena, Italy; Istituto di Microbiologia, Università'La Sapienza', Piazzale A. Moro, 5, 00185 Rome, Italy; Istituto di Microbiologia, UniversitàCattolica 'S. Cuore', Largo F. Vito, 1, 00168 Rome, Italy |
| Abstract: | Abstract An acid phosphatase containing a 27-kDa polypeptide component has been identified in Escherichia coli by means of a zymogram technique. The enzyme is secreted in the periplasmic space and is able to hydrolyze several organic phosphate esters, but not diesters, showing preferential activity on p -nitrophenyl phosphate and other phenolic phosphate esters. Production of the enzyme apparently occurs only in cells growing on carbon sources other than glucose. |
| Keywords: | Escherichia coli Acid phosphatase |