Isolation,identification and characterization of <Emphasis Type="Italic">Bacillus subtilis</Emphasis> ZJB-063, a versatile nitrile-converting bacterium |
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Authors: | Yu-Guo Zheng Jing Chen Zhi-Qiang Liu Ming-Huo Wu Liang-Ying Xing Yin-Chu Shen |
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Institution: | (1) Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China |
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Abstract: | Strain ZJB-063, a versatile nitrile-amide-degrading strain, was newly isolated from soil in this study. Based on morphology,
physiological tests, Biolog and the 16S rDNA sequence, strain ZJB-063 was identified as Bacillus subtilis. ZJB-063 exhibited nitrilase activity without addition of inducers, indicating that the nitrilase in B. subtilis ZJB-063 is constitutive. Interestingly, the strain exhibited nitrile hydratase and amidase activity with the addition of
ɛ-caprolactam. Moreover, the substrate spectrum altered with the alteration of enzyme systems due to the addition of ɛ-caprolactam.
The constitutive nitrilase was highly specific for arylacetonitriles, while the nitrile hydratase/amidase in B. subtilis ZJB-063 could not only hydrolyze arylacetonitriles but also other nitriles including some aliphatic nitriles and heterocyclic
nitriles. Despite comparatively low activity, the amidase of hydratase/amidase system was effective in converting amides to
acids. The versatility of this strain in the hydrolysis of various nitriles and amides makes it a potential biocatalyst in
organic synthesis. |
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Keywords: | Bacillus subtilis Isolation Identification Nitrilase Nitrile hydratase Substrate spectrum |
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