Lipopeptides Produced by a Soil <Emphasis Type="Italic">Bacillus Megaterium</Emphasis> Strain |
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Authors: | Manuel Troyano Pueyo Jr" target="_blank">Carlos BlochJr Ana Maria Carmona-Ribeiro Paolo di Mascio |
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Institution: | 1.Departamento de Bioquimica, Instituto de Quimica,Universidade de S?o Paulo,S?o Paulo,Brazil;2.Laboratório de Espectrometria de Massa, EMBRAPA,Recursos Genéticos e Biotecnologia,Brasília,Brazil;3.S?o Paulo,Brazil |
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Abstract: | A soil microorganism identified as Bacillum megaterium was found to produce several antibiotics substances after growth for 20 h at 37°C in a mineral culture medium. Analysis both
by electron spray ionization (ESI) and matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry
(MS) identified these substances as lipopeptides. Predominant peaks at m/z 1,041 and m/z 1,065 revealed ions which are compatible with surfactins and lichenysins, respectively. Two other ions m/z 1,057 and m/z 1,464 were further studied by collision-induced dissociation (CID) unveiling an iturin A at the first and fengycins A and
B at the second m/z peaks. The CID spectrum of the m/z 1,464 ion also suggests the existence of fengycins A and B variants in which Ile was changed to Val in the position 10 of
the peptide moiety. Raw mixtures of all these compounds were also assayed for antibiotic features. The data enlighten the
unusual diversity of the lipopeptide mixture produced by a sole Bacillus species. |
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