Trypanosoma cruzi: location of a specific antigen on the surface of bloodstream trypomastigote and culture epimastigote forms. |
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Authors: | J Fruit D Afchain A Petitprez A Capron |
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Affiliation: | 1. Centre d''Immunologie et de Biologie Parasitaire, Institut Pasteur, 20 boulevard Louis XIV, 59012, Lille Cédex, France;2. Service de Parasitologie, U.E.R. de Pharmacie, Lille, France |
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Abstract: | The nature of surface antigens of culture epimastigote and bloodstream trypomastigote forms of Trypanosoma cruzi was investigated by light and electron microscopy using indirect immunofluorescence and peroxidase labeling techniques and antisera against unique, common, and contaminant antigens. A specific antigen, identified by monospecific rabbit antiserum (anti-component 5 antiserum), is the major constituent of the cell surface and flagellar membrane of both the culture epimastigote and bloodstream trypomastigote forms. Antigens of heterologous stercorarian trypanosomes (Trypanosoma rangeli) and of culture medium proteins could not be detected on the cell surface of culture epimastigote forms and bloodstream trypomastigote forms. |
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Keywords: | Protozoa parasitic Epimastigote culture form Trypomastigote Immunology Antigenicity Surface antigens localization Immunofluorescence tests Immunoperoxidase tests Electron microscopy Mouse Culture |
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