Plant Bax Inhibitor-1 interacts with ATG6 to regulate autophagy and programmed cell death |
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| Authors: | Guoyong Xu Shanshan Wang Shaojie Han Ke Xie Yan Wang Jinlin Li |
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| Institution: | 1. MOE Key Laboratory of Bioinformatics, Center for Plant Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China;2. Department of Biology, Duke University, Durham, NC, USA;3. Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, USA;4. School of Chemistry and Biological Engineering, University of Science and Technology, Beijing, China |
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| Abstract: | Autophagy is an evolutionarily conserved catabolic process and is involved in the regulation of programmed cell death during the plant immune response. However, mechanisms regulating autophagy and cell death are incompletely understood. Here, we demonstrate that plant Bax inhibitor-1 (BI-1), a highly conserved cell death regulator, interacts with ATG6, a core autophagy-related protein. Silencing of BI-1 reduced the autophagic activity induced by both N gene-mediated resistance to Tobacco mosaic virus (TMV) and methyl viologen (MV), and enhanced N gene-mediated cell death. In contrast, overexpression of plant BI-1 increased autophagic activity and surprisingly caused autophagy-dependent cell death. These results suggest that plant BI-1 has both prosurvival and prodeath effects in different physiological contexts and both depend on autophagic activity. |
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| Keywords: | ATG6 autophagy Bax inhibitor-1 programmed cell death Tobacco mosaic virus |
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