Secretogranin II Is Synthesized and Secreted in Astrocyte Cultures |
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| Authors: | Reiner Fischer-Colbrie Rudolf Kirchmair Angelika Schobert Claudia Olenik Dieter K Meyer Hans Winkler |
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| Institution: | Department of Pharmacology, University of Innsbruck, Innsbruck, Austria;Department of Pharmacology, University of Freiburg, Freiburg, F.R.G. |
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| Abstract: | Abstract: Astrocyte cultures from rat brain were analyzed for their ability to synthesize and secrete secretogranin II (chromogranin C). Northern blot analysis of polyA-selected RNA established the presence of secretogranin II mRNA in these cells. By radioimmunoassay, 11.6 fmol/106 astrocytes of secretogranin II was found in these cells. About twice the amount was released into the medium within 3 days. Secretogranin II within the astrocytes was practically unprocessed, as shown by HPLC. These results establish for the first time that astrocytes in vitro synthesize and sec rete a protein of the acidic chromogranin family. |
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| Keywords: | Chromogranins Carboxypeptidase H Secretoneurin |
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