Serological Relationships Among the Over-expressed Coat Proteins of Allexiviruses |
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| Authors: | Y-W Lu J Chen H-Y Zheng M J Adams and J-P Chen |
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| Institution: | Authors' addresses: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;;Plant Pathology and Microbiology Department, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK (correspondence to J.-P. Chen. E-mail: ) |
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| Abstract: | Coat protein genes of six members of the genus Allexivirus, Garlic viruses ‐A, ‐B, ‐C, ‐D, ‐E and ‐X, were over‐expressed in bacteria and used to prepare polyclonal antibodies. In western blot analysis, Garlic virus D (GarV‐D) and GarV‐E were closely related serologically and there were weaker relationships between some other pairs, particularly GarV‐E with GarV‐A, GarV‐X with GarV‐A, GarV‐X with GarV‐B and GarV‐X with GarV‐C. Sequence analysis suggested that there was no simple relationship between amino acid identity and serological properties. |
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| Keywords: | allexiviruses coat protein serological relationship |
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