Two distinct cis-acting elements are involved in light-dependent activation of the pea elip promoter |
| |
Authors: | Jens Blecken Bernd Weisshaar and Frank Herzfeld |
| |
Institution: | (1) Institut für Botanik der Universität Hannover, Herrenhäuser StraBe 2, 30419 Hannover, Germany;(2) Max Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Carl von Linné Weg 10, 50829 Köln, Germany |
| |
Abstract: | Light activation of the pea (Pisum sativum) elip gene promoter was analysed in transgenic plants and in transiently transfected plant protoplasts. A series of promoter deletions fused to the gusA reporter was tested, and the results obtained by the two experimental approaches were in good agreement. We identified two nucleotide sequence elements involved in light-regulated expression of the elip gene. One element is similar to the GT1 binding site of the rbcS-3A gene, and the other resembles a G-box-like ACGT element. The region containing both elements was able to confer light responsiveness on a heterologous basic promoter. Electrophoretic mobility shift assays demonstrated that each element is specifically recognized by DNA-binding proteins present in nuclear extracts from pea seedlings. The G-box-like ACGT element is necessary but not sufficient for light inducibility, indicating that the two elements act together in confering light responsiveness. |
| |
Keywords: | ELIP Light induction Transient expression UV-light Light regulatory unit |
本文献已被 SpringerLink 等数据库收录! |
|