Screening for Booroola (FecB) and Galway (FecXG) mutations in Indian sheep |
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| Affiliation: | 1. Animal Biotechnology Section, Central Sheep and Wool Research Institute, Avikanagar, Rajasthan 304501, India;2. Animal Genetics and Breeding Division, Central Sheep and Wool Research Institute, Avikanagar, Rajasthan 304501, India;3. Animal Nutrition Division, Central Sheep and Wool Research Institute, Avikanagar, Rajasthan 304501, India;4. Department of Animal Breeding and Genetics, Orissa Veterinary College, Orissa University of Agriculture & Technology, Bhubaneswar 751003, India;1. Division of Veterinary Biotechnology, ICAR-IVRI, Izatnagar, Bareilly, UP, 243122, India;2. Division of Biological Products, ICAR-IVRI, Izatnagar, Bareilly, UP, 243122, India;3. Division of Veterinary Pathology, ICAR-IVRI, Izatnagar, Bareilly, UP, 243122, India;4. Division of Virology, ICAR-IVRI, Mukteshwar Campus, Nainital, 263138, India;5. Division of Biological Standardization, ICAR-IVRI, Izatnagar, Bareilly, UP, 243122, India;1. ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, 560030, Karnataka, India;2. Academy of Climate Change Education and Research, Kerala Agricultural University, Vellanikkara, Thrissur, Kerala, India;1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China;2. Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou, China;3. Sheep Breeding Biotechnology Engineering Laboratory of Gansu Province, Minqin, 733300, China;1. Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA)- Instituto Agroalimentario de Aragón (IA2) (CITA-Universidad de Zaragoza), Zaragoza, Spain;2. ARAID, Zaragoza, Spain;3. Université de Toulouse, INRA, ENVT, GenPhySE, Castanet-Tolosan, France;4. Departamento de Mejora Genética Animal, INIA, Madrid, Spain;1. ICAR- Central Sheep and Wool Research Institute, Avikanagar (via Jaipur), Rajasthan 304501, India;2. ICAR-National Dairy Research Institute, Karnal, Haryana 132001, India;1. Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran;2. Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran;3. Departamento de Producción Animal, Facultad de Veterinaria, Avda., Puerta de Hierro s/n, E-28040 Madrid, Spain |
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| Abstract: | This study reports the status of the Booroola (FecB) and Galway (FecXG) mutations in Indian sheep breeds. The Kendrapada sheep (n = 46) was genotyped for the presence of FecB and FecXG mutations, while the Garole (n = 34), Malpura (n = 30), and Decanni sheep (n = 15) for the FecXG mutation. The FecB and FecXG genotyping was carried out by forced restriction fragment length polymorphism PCR technique. In the present study, FecB mutation was discovered in the Kendrapada sheep of Orissa, which is now the second prolific sheep of India after the Garole. Out of 46 individuals of Kendrapada sheep, 26 were homozygous (BB), 15 heterozygous (B+) and 5 non-carriers (++) for the FecB mutation. The frequency of the FecB allele in this sample was about 0.73. Results indicated that the frequency of the FecB mutation is high, but the gene is not fixed in the population as reported in Garole sheep. None of sheep breeds carried the FecXG mutation. The discovery of the FecB mutation in Kendrapada sheep will facilitate the use of FecB allele in improving the prolificacy of non-prolific sheep breeds of India. |
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