普通冠状病毒229E株的分子流行病学分析

目的明确哈尔滨地区普通冠状病毒229E(Human Coronavirus-229E，HCOV-229E)株的流行和变异情况及其与SARS-CoV的异同，为进一步掌握该地区常见上呼吸道病毒的流行规律及预防，乃至疫苗的制备打下基础。方法利用RT—PCR法对2003年上半年采集的部分发热病人血清及血细胞进行筛选，同时采用基因测序、序列分析等手段对扩增的HCOV-229E N gene片断进行蛋白和基因分析。结果55例标本中HCOV-229E RNA阳性病例5例，占9．09％；测序结果看出哈尔滨地区检出229E株N基因的序列与已公布的HCOV-229E Ngene(295—802)序列完全相同，所测片断为HCOV-229E N gene的部分序列。该基因与猪流行性腹泻病毒、犬冠状病毒、TGEV及猫感染性腹膜炎病毒有一定同源性；但与已公布的SARS-CoV N gene序列比较，相似性小于1％。结论(1)该地区发热病人普通冠状病毒229E株阳性率为9．09％；(2)哈尔滨地区流行的HCOVN基因无变异发生；(3)HCOV-229E与猪流行性腹泻病毒、犬冠状病毒、TGEV和猫感染性腹膜炎病毒有一定的同源性；(4)哈尔滨地区HCOV-229E N gene与SARS-CoV N gene相似性小于1％。

The screening and identification of Human Coronavirus-229E

Objective To know the epidemic condition and the variation of HcoV-229E in Harbin,to analyse the difference between HcoV-229E and SARS-CoV. Methods We adaped RT-PCR to detect HcoV-229E RNA,and we adapted gene sequencing method to get HcoV-229E N gene sequence.Results We detected 55 samples,and found 5 positive cases,the positive percent was 9.09%;The analysis of sequence showed that The sequence of Harbin was the same to HcoV-229E N gene which had been published,the gene segment was part of HcoV-229E N gene;The sequencing result had less 1% similarity with SARS-CoV N gene.Conclusions (1) The positive percent of HcoV-229E in Harbin was 9.09%,there was coincidence results through the RT-PCR method and blood serum epidemiology method;(2) The HcoV-229E in Harbin had no variation;(3) There were homology between HcoV-229E and porcine epidemic diarrhea virus,Canine coronavirus,Transmissible gastroenteritis virus,feline infectious peritonitis virus;(4) The similarity of HcoV-229E N gene in Harbin and SARS-CoV N gene was less than 1%,the result accords with correlated report.