十二烷基苯磺酸钠对引发内置物感染表皮葡萄球菌生物被膜的作用

【背景】随着医用内置物的广泛使用,由表皮葡萄球菌生物被膜导致的医院获得性感染不断增多,目前鲜见关于表面活性剂针对表皮葡萄球菌生物被膜作用的报道。【目的】通过研究阴离子型表面活性剂十二烷基苯磺酸钠(sodium dodecyl benzene sulfonate,SDBS)分别对ATCC 35984 (产膜表皮葡萄球菌标准株)生物被膜的清除、生物被膜内细菌代谢和形成生物被膜的关键物质多糖胞间黏附素(polysaccharide intercellular adhesion,PIA)产生的影响,为临床使用SDBS防治由表皮葡萄球菌生物被膜引起的相关感染提供可靠的理论及实践依据。【方法】利用XTT减低法,评价SDBS对ATCC 35984已形成生物被膜的清除效率及对生物被膜内细菌代谢的影响;激光共聚焦显微镜观察SDBS对生物被膜作用的效果;采用刚果红培养基观察SDBS对PIA产生的影响。【结果】浓度为256、128、64、32、16 mg/L的SDBS在作用6、12、24 h时,对ATCC 35984的生物被膜均有显著的清除效率(P<0.01);浓度为32 mg/L时对生物被膜内细菌的...

Effect of sodium dodecyl benzene sulfonate on biofilm of Staphylococcus epidermidis with infection of the implant

[Background] With the widespread use of medical implants, hospital-acquired infections caused by Staphylococcus epidermidis biofilm are increasing. For now, there are few reports about the effect of surfactant on the biofilm of Staphylococcus epidermidis. [Objective] To demonstrate the effect of anionic surfactant sodium dodecyl benzene sulfonate, including scavenging ATCC 35984 biofilm, bacterial metabolism and formation of polysaccharide intercellular adhesion (PIA). To provide reliable theory and practical evidence for SDBS, and prevent related infections caused by Staphylococcus epidermidis biofilm in clinic. [Methods] XTT reduction method was used to explore the effect of SDBS on the clearance of ATCC 35984 biofilm and bacterial metabolism in the biofilm. Laser scanning confocal microscope (LSCM) and Congo red medium was respectively used to observe the effect of SDBS on biofilm and PIA. [Results] SDBS on the concentration of 256 mg/L, 128 mg/L, 64 mg/L, 32 mg/L, 16 mg/L has significantly clearance on ATCC 35984 in the 6 h, 12 h, 24 h, respectively (P<0.01). It is significantly inhibition effect of SDBS with 32 mg/L on bacterial metabolism in the biofilm (P<0.05). 256 mg/L, 128 mg/L, 64 mg/L SDBS has satisfied clearance. 64 mg/L and 32 mg/L SDBS has no inhibition effect on PIA formation under the LSCM observation. [Conclusion] SDBS has significant inhibition effect on bacterial metabolism in the biofilm of Staphylococus epidermidis and damage the morphological structure of biofilm.