| 艰难梭菌基因编辑技术研究进展 |
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| 引用本文： | 洪伟,万雯,崔古贞,官志忠,齐晓岚,禹文峰.艰难梭菌基因编辑技术研究进展[J].生物工程学报,2020,36(2):210-225. |
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| 作者姓名： | 洪伟 万雯 崔古贞 官志忠 齐晓岚 禹文峰 |
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| 作者单位： | 1贵州医科大学 地方病与少数民族疾病教育部重点实验室，贵州 贵阳 550004;2贵州省医学分子生物学重点实验室，贵州 贵阳 550004,3贵州医科大学 病理学教研室，贵州 贵阳 550004,4贵州医科大学 基础医学院 微生物教研室，贵州 贵阳 550004,1贵州医科大学 地方病与少数民族疾病教育部重点实验室，贵州 贵阳 550004;2贵州省医学分子生物学重点实验室，贵州 贵阳 550004,1贵州医科大学 地方病与少数民族疾病教育部重点实验室，贵州 贵阳 550004;2贵州省医学分子生物学重点实验室，贵州 贵阳 550004,1贵州医科大学 地方病与少数民族疾病教育部重点实验室，贵州 贵阳 550004;2贵州省医学分子生物学重点实验室，贵州 贵阳 550004 |
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| 基金项目： | 国家自然科学基金 (Nos. 31560318，31601012，31760318，U1812403)，贵州省科技计划项目黔科合平台人才 (No. [2017]5652) 资助。 |
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| 摘 要： | 艰难梭菌Clostridioesdifficile是一种革兰氏阳性、产芽孢、专性厌氧细菌,是医院相关性腹泻的主要病原体。近年来,随着强毒株的出现(如核糖体027型),其流行性与致死率逐年上升,因此对艰难梭菌生理、生化特征及致病机制的研究受到广泛重视。艰难梭菌生理、生化特征及致病机制研究又以建立其稳定、高效的基因编辑方法为必要前提。借助基因编辑工具,研究者可以扰动艰难梭菌核心生物学过程,在分子水平研究其分子致病机制。如Clos Tron技术在艰难梭菌毒素A (Toxin A)和毒素B (Toxin B)与其致病力关系的研究中起到了关键作用。文中以时间为主线综述了艰难梭菌基因编辑技术的发展历程和最新进展,并对艰难梭菌基因编辑技术未来的研究方向进行展望。
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| 关 键 词： | 艰难梭菌 艰难梭菌感染 基因编辑 基因功能研究 致病机制 |
| 收稿时间： | 2019/5/2 0:00:00 |
Advances in Clostridioes difficile genome editing |
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| Wei Hong,Wen Wan,Guzhen Cui,Zhizhong Guan,Xiaolan Qi and Wenfeng Yu.Advances in Clostridioes difficile genome editing[J].Chinese Journal of Biotechnology,2020,36(2):210-225. |
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| Authors: | Wei Hong Wen Wan Guzhen Cui Zhizhong Guan Xiaolan Qi and Wenfeng Yu |
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| Institution: | 1 Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, Guizhou, China;2 Key Laboratory of Medical Molecular Biology, Guizhou Province, Guiyang 550004, Guizhou, China,3 Department of Pathology, Guizhou Medical University, Guiyang 550004, Guizhou, China,4 Department of Microbiology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550004, Guizhou, China,1 Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, Guizhou, China;2 Key Laboratory of Medical Molecular Biology, Guizhou Province, Guiyang 550004, Guizhou, China,1 Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, Guizhou, China;2 Key Laboratory of Medical Molecular Biology, Guizhou Province, Guiyang 550004, Guizhou, China and 1 Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, Guizhou, China;2 Key Laboratory of Medical Molecular Biology, Guizhou Province, Guiyang 550004, Guizhou, China |
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| Abstract: | Clostridioes difficile is a Gram-positive, spore-forming, obligate anaerobic bacterium, and the main cause of hospital-associated diarrhea. In recent years, with the presence of virulent strains (i.e., ribosome type 027), the prevalence and mortality events have increased. Thus, studies on physiological and biochemical characteristics, and pathogenic mechanisms of C. difficile have been performed. The development of efficient and stable genome-editing methods for C. difficile is urgent for the dissection of its physiological and pathogenic mechanism. For example, ClosTron technology plays a key role in study of the relationship between C. difficile toxins (Toxin A and Toxin B) and its pathogenicity. This article reviews the history, recent progress and future prospects of C. difficile genome-editing technologies. |
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| Keywords: | Clostridioes difficile Clostridioes difficile infection (CDI) genome editing gene function research pathogenic mechanism |
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