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Immobilized laccase for decolourization of Reactive Black 5 dyeing effluent
Authors:Andrea Zille  Tzanko Tzanov  Georg M Gübitz  Artur Cavaco-Paulo
Institution:(1) Department of Textile Engineering, University of Minho, 4800-058 Guimarães, Portugal;(2) Department of Environmental Biotechnology, Graz University of Technology, 8010 Graz, Austria
Abstract:Reactive Black 5 industrial dyeing effluent was decolourized by free and immobilized laccase. The stability of the enzyme (194 h free and 79 h immobilized) depended on the dyeing liquor composition and the chemical structure of the dye. In the decolourization experiments with immobilized laccase, two phenomenons were observed – decolourization due to adsorption on the support (79%) and dye degradation due to the enzyme action (4%). Dyeing in the enzymatically recycled effluent provided consistency of the colour with both bright and dark dyes.
Keywords:decolourization  laccase  recycling of dyeing effluents  stability
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