Restriction map of Tn7 |
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Authors: | Françoise Gosti-Testu Victor Norris Jean Brevet |
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Institution: | Institut de Microbiologie, Bât. 409 Université de Paris-Sud, 91405 Orsay Cédex, France |
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Abstract: | Tn7, a transposon of 14 kb, encodes resistance to trimethoprim (Tp) and streptomycin (Sm). A cleavage site map of this transposon for twenty-two different restriction enzymes as determined by comparison of restriction enzyme cleavage patterns of the plasmids ColE1 and ColE1::Tn7 is presented. The precise localization of these sites was facilitated by the use of two deletion derivatives of ColE1::Tn7: pGB2 and ColE1::Tn7Δ6, and by the use of pOB14 and pOB15 which contain a part of Tn7 cloned into the plasmid pBR322. This map should aid in the study of the structural and genetic organization of this transposon. |
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