| 重组肺炎链球菌表面黏附素A(rPsaA)的融合表达及抗原性分析 |
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| 引用本文： | 何斌,朱虹,端青.重组肺炎链球菌表面黏附素A(rPsaA)的融合表达及抗原性分析[J].微生物学免疫学进展,2007,35(2):12-14. |
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| 作者姓名： | 何斌 朱虹 端青 |
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| 作者单位： | 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京,100071 |
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| 摘 要： | 为探索一种大量表达功能性重组肺炎链球菌表面黏附素A(rPsaA)的方法,用PCR和基因重组技术将基因psaA的先导序列替换为伯氏疏螺旋体外膜蛋白A(OspA)信号肽基因序列,并在大肠杆菌中表达。表达产物用SDS-PAGE检测,Western blot分析抗原性。结果表明,rPsaA表达量明显提高且有较好的抗原性。
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| 关 键 词： | 重组肺炎链球菌表面黏附素A 融合表达 抗原性 |
| 文章编号： | 1005-5673（2007）02-0012-03 |
| 收稿时间： | 2006-11-22 |
| 修稿时间： | 2006-11-222007-01-18 |
Fusion expression and antigenicity analysis of recombinant pneumococcal surface adhesin A (rPsaA) |
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| HE Bin,ZHU Hong,DUAN Qing.Fusion expression and antigenicity analysis of recombinant pneumococcal surface adhesin A (rPsaA)[J].Progress In Microbiology and Immunology,2007,35(2):12-14. |
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| Authors: | HE Bin ZHU Hong DUAN Qing |
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| Institution: | Institute of Microbiology and Epidemiology ,Academy of Military Medical Science, State key laboratory of pathogen and biosecurity. Beefing 100071 ,China |
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| Abstract: | To search for a new method to achieve greater levels of expression and production of large quantities of functional rPsaA,the native leader sequence of gene psaA was replaced with B.burgdorferi ospA signal sequence by PCR and genetic recombinant technique,and expressed in Escherichia coli.The expressed protein was analysed by SDS-PAGE and Western blot.Results showed that rPsaA is antigenic and can achieve high level in expression. |
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| Keywords: | Recombinant PsaA Fusion expression Antigenicity |
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