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The reaction centre from green sulphur bacteria: progress towards structural elucidation
Authors:Rémigy  Hervé-W  Hauska  Günter  Müller  Shirley A  Tsiotis  Georgios
Institution:(1) Division of Biochemistry, Department of Chemistry, University of Crete, P.O. Box 1470, 71409 Heraclion, Greece;(2) M. E. Müller Institute for Microscopic Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland;(3) Lehrstuhl für Zellbiologie und Pflanzenphysiologie, University of Regensburg, 93040 Regensburg, Germany
Abstract:The reaction centre (RC) of green sulphur bacteria is a FeS-type RC, as are the RCs of Photosystems I (PS I) of oxygenic photosynthetic organisms and of heliobacteria. The core domains of both green sulphur bacterial and heliobacterial RCs are considered to be homodimeric, in contrast to those of purple bacteria, PS I and Photosystem II (PS II). This paper briefly describes the techniques of electron microscopy and image processing suited to investigate the structure of these proteins. Recent advances in the study of the structure of the green sulphur bacterial RC, primarily achieved by the application of scanning transmission electron microscopy, are reviewed.This revised version was published online in October 2005 with corrections to the Cover Date.
Keywords:Chlorobium tepidum  green sulphur bacteria  reaction centre  scanning transmission electron microscopy
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