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Alteration of mitochondrial DNA sequence and copy number in nasal polyp tissue
Authors:Sang-Young Park  Myung-Geun Shin  Hye-Ran Kim  Ji-Yeon Oh  Soo-Hyun Kim  Jong-Hee Shin  Yong-Bum Cho  Soon-Pal Suh  Dong-Wook Ryang
Institution:1. Department of Laboratory Medicine and Molecular Genetics, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, South Korea;2. Department of Otorhinolaryngology-Head and Neck Surgery, Chonnam National University Medical School and Chonnam National University Hospital, Gwangju, South Korea;3. Genome Research Center for Hematopoietic Disease, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, South Korea;4. Brain Korea 21 Project, Center for Biomedical Human Resources, Chonnam National University, Gwangju, South Korea;1. XuZhou Children''s Hospital, 18, Sudi North Road, Xuzhou, Jiangsu Province 221006, PR China;2. Department of Anesthesiology, East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China;3. Department of Anesthesiology, Jinan Central Hospital, Shandong University School of Medicine, Jinan 250013, PR China;4. Anesthesiology Department, The First Hospital of Shijiazhuang City, 36 Fanxi Road, Chang’an District, Shijiazhuang 050011, PR China;5. Department of Anesthesiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, PR China;6. Department of Anesthesiology, Jiangsu Provincial Hospital of Integrated Chinese Traditional and Western Medicine, 100, Shizi Street, Hongshan Road, Nanjing 210028, PR China;7. Department of Anesthesiology, The Ninth People''s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, PR China;1. Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, DHHS, Bethesda, MD, USA;2. Scientific Applications International Corporation, Frederick, MD, USA;3. Department of Obstetrics, Gynaecology & Perinatology, Obafemi Awolowo University, Ile-Ife, Nigeria;4. Albert Einstein College of Medicine, Yeshiva University, The Bronx, NY, USA;1. Environmental Toxicology Program, Clemson University, Clemson, SC, United States;2. Department of Biological Sciences, Clemson University, Clemson, SC, United States
Abstract:This study was designed to investigate the possibility that mtDNA mutations might arise in inflammatory or chronically damaged nasal polyp tissue from 23 patients. Thirteen patients (57%) displayed nasal polyp tissue-specific mtDNA mutations in the hypervariable segment of the control region and cytochrome b gene, which were not found in the corresponding blood cells and/or adjacent normal tissue. Nasal polyp tissue-specific length heteroplasmic mutations were also detected in nucleotide position (np) 303–315 homopolymeric poly C track (39%), np 514–523 CA repeats (17%) and np 16184–16193 poly C track (30%). The average mtDNA copy number was about three times higher in nasal polyp tissue than in the corresponding peripheral blood cells and adjacent non-polyp tissues. The level of reactive oxygen species (ROS) was significantly higher in the nasal polyp tissues compared to those from the corresponding samples. High level of ROS in nasal polyp tissue may contribute to development of mtDNA mutations, which may play a crucial role in the vicious cycle of pathophysiology of nasal polyps.
Keywords:Nasal polyp  mtDNA  Heteroplasmic mutation  Copy number  ROS
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