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| 骨髓基质干细胞的分离纯化及培养 | |
| 引用本文： | 阮绪芝,陈霞萍,严世荣,王卫民. 骨髓基质干细胞的分离纯化及培养[J]. 生物学杂志, 2004, 21(2): 25-27 |
| 作者姓名： | 阮绪芝 陈霞萍 严世荣 王卫民 |
| 作者单位： | 1. 郧阳医学院,生物学教研室,十堰,442000 2. 郧阳医学院,附属太和医院生命科学研究所,十堰,442000 3. 郧阳医学院,生物化学教研室,十堰,442000 |
| 摘 要： | 目的 建立骨髓基质干细胞(MSCs)良好的分离纯化和培养方法。方法 将小鼠骨髓基质干细胞自殷骨中分离，应用贴壁选择法结合细胞克隆挑选法进行分离纯化，应用细胞生长因子(EGF和PDGF-BB)刺激法进行MSCs的体外培养和传代，倒置显微镜下观察分离培养的细胞并照像记录。结果，培养获得了纯化的呈梭形成纤雏样细胞的骨髓基质干细胞。在生长因子EGF和PDGF-BB的共同作用下，传代MSCs生长旺盛，形态均一。结论 该方法是简便高效的骨髓基质干细胞的分离纯化和培养方法。 |
| 关 键 词： | 小鼠 骨髓基质干细胞 细胞培养 |
| 文章编号： | 1008-9632(2004)02-0025-03 |
| 修稿时间： | 2003-12-08 |
Preparation and culture of murine bone marrow stromal stem cdlls |
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| RUAN Xu_zhi CHEN Xia_ping YAN Shi_rong Wang Wei_min. Preparation and culture of murine bone marrow stromal stem cdlls[J]. Journal of Biology, 2004, 21(2): 25-27 | |
| Authors: | RUAN Xu_zhi CHEN Xia_ping YAN Shi_rong Wang Wei_min |
| Affiliation: | RUAN Xu_zhi~1 CHEN Xia_ping~2 YAN Shi_rong~3 Wang Wei_min~2 |
| Abstract: | A method for isolating and culturing mouse bone marrow_detived mesenchymal stem cells (mMSCs) were established.MSCs were isolated from mouse thighbone marrow and cultured in Dulbecco's modified eagle's medium with low glucose and purified by wall_sticking and colonies collection,and were proliferated through subculturing by adding epidermal growth factor (EGF)and pletelet_derived growth factor(PDGF-BB).Cellular proliferation were observed and recorded under the inverted microscope.Purified MSCs were acquired and became fibroblast_like.EGF and PDGF_BB were effective agents for mMSCs proliferation in vitro. |
| Keywords: | murine bone marrow stromal stem cells cell culture. |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |