Metal affinity extraction of human hemoglobin in an aqueous polyethylene glycol-sodium sulfate two-phase system |
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Authors: | S D Plunkett F H Arnold |
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Institution: | (1) Division of Chemistry & Chemical Engineering 210-41, California Institute of Technology, 91125 Pasadena, CA, USA |
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Abstract: | Summary We have developed a protein extraction technique which uses metal affinity ligands in PEG/salt aqueous two-phase systems. Cu(II)IDA-PEG will partition proteins according to their surface histidine contents in two-phase systems formed from sodium sulfate and polyethylene glycol. The nearly complete separation of human hemoglobin and human serum albumin in a single stage is presented as a demonstration of the effectiveness of metal affinity extraction in PEG/salt systems. |
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