Recombination between satellite phage P4 and its helper P2. II. In vitro construction of a helper-independent P4: :P2 hybrid phage |
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| Authors: | B H Lindqvist |
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| Affiliation: | Institute of Medical Biology, University of Tromsø, 9001 Tromsø Norway |
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| Abstract: | A helper-independent P4::P2 hybrid (Hy19), with the essential gene region of P4 linked to the late genes of P2, has been isolated by in vitro recombination techniques. This hybrid expresses a P4 Sid? phenotype since it makes large heads. The int-C region of P2 is deleted from Hy19 and its DNA replication is independent of the host rep gene, indicating that it depends on the P4 replicon. |
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| Keywords: | Recombinant DNA restriction enzyme |
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