Water-soluble glycoproteins from Cannabis sativa (South Africa) |
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Authors: | Agnes Hillestad Jens K Wold |
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Institution: | Department of Pharmacognosy, Institute of Pharmacy, University of Oslo, Oslo 3, Norway |
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Abstract: | The water-soluble glycoproteins obtained from Cannabis leaves of plants grown from South African seeds have been further studied. Treatment of the glycoprotein fractions with NaOH in the presence of NaBH4, resulted in a significant decrease in the serine content and a corresponding increase in alanine. The carbohydrate side chains released contained the sugar alcohol, galactitol. By treatment of the glycoprotein fractions with NaOH in the presence of Na2SO3, and subsequent acid hydrolysis, cysteic acid was formed. These data indicate that carbohydrate and protein are connected via serine-O-galactoside linkages. Further investigation of the structure of the carbohydrate part of the glycoproteins was carried out by methylation analysis, Smith-degradation and enzyme incubation. The present glycoprotein material of plants grown from South African seeds is similar to the material previously investigated, but in contrast to the latter, it is devoid of hexosamine. |
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Keywords: | Cannabinaceae marijuana glycoprotein cell wall protein hydroxyproline |
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