Intracellular localisation of hexokinase in Cuscuta reflexa |
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Authors: | Madhulika Baijal GG Sanwal |
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Institution: | Department of Biochemistry, University of Lucknow, Lucknow, U.P., India |
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Abstract: | In Cuscuta reflexa 16% of the hexokinase activity was associated with the particulate fraction and the rest in the 105 000 g, 1 hr supernatant. In a sucrose gradient, hexokinase activity banded with an organelle at a mean density of 1.20 g cm?3, coinciding with the mitochondrial marker, cytochrome c oxidase. Fractionation of isolated mitochondria by digitonin showed the presence of the enzyme in the outer membrane along with its marker rotenone-insensitive NADH cytochrome c reductase. No latent form of hexokinase was detected. |
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Keywords: | Convolvulaceae angiospermic parasite hexokinase phosphotransferase |
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