Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe |
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| Authors: | Jun Long Xing‐ming Wang Dong‐ling Xu Li‐sheng Ding |
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| Affiliation: | 1. School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang, China;2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China |
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| Abstract: | Under the condition of physiological pH environment (pH = 7.40), the interactions of safranin T (ST) with herring sperm DNA were studied by means of spectral methods using acridine orange (AO) as a fluorescence probe. The spectroscopic characteristics of DNA–AO in the case of ST (along with the increase of concentration) were observed in an aqueous medium. The binding constants for ST stranded DNA and competitive bindings of ST interacting with DNA–AO systems were examined by fluorescence spectra, and the binding mechanism of ST with DNA was researched via viscosity measurements. All the testimony manifested that bonding modes between ST and DNA were evidenced to be intercalative binding and electrostatic binding, and the combining constant of ST with DNA was obtained. The binding of ST to DNA was driven by entropy and enthalpy through the calculated thermodynamic parameters (ΔrHm?, ΔrSm and ΔrGm?). Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | safranin T fluorescence spectra DNA interaction acridine orange |
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