Alpha-Phenyl-N-Tert-Butylnitrone Attenuates Excitotoxicity in Rat Striatum by Preventing Hydroxyl Radical Accumulation |
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Authors: | Eric Lancelot Marie-Louise Revaud Roger G Boulu Michel Plotkine Jacques Callebert |
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Affiliation: | a Laboratoire de Pharmacologie, Universite Rene Descartes, 75270 Paris Cedex 06, France |
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Abstract: | Various in vitro experiments have indicated that oxygen-derived free radicals may contribute to excitotoxic neuronal death. In the present study we induced excitotoxicity in rat striatum by perfusing glutamate at a high concentration through a microdialysis probe. We observed an increased formation of hydroxyl radicals (˙OH) during the perfusion of the excitotoxin and an extensive striatal lesion 24 h after the insult. The spin trap, -phenyl-N-tert-butylnitrone (PBN), attenuated both hydroxyl radical levels and the volume of the lesion. This result suggests that the neuroprotection may be due to a free radical scavenging mechanism. It also implies that PBN may be used in pathological situations involving excitotoxicity such as stroke, brain trauma, and chronic neurologic diseases. |
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Keywords: | oxidative stress toxicity hydroxyl radical n tert butyl alpha phenylnitrone |
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