Development of an apparatus for cultivation of anaerobic microorganisms |
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| Authors: | H. Tanaka M. Nakanishi J. C. Ogbonna Y. Ashihara |
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| Affiliation: | (1) Institute of Applied Biochemistry, University of Tsukuba, 305 Tsukuba, Ibaraki, Japan |
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| Abstract: | Summary A novel apparatus with an L-shaped test tube was developed for anaerobic cell cultivation. Anaerobic condition was achieved without the rigorous gassing with CO2 during the various stages of medium preparation. dispensation and cell inoculation. The growth of both moderate and strict anaerobes in this apparatus were similar to those obtained with the glove box method. |
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