Anaerobic degradation of cyclohexane-1,2-diol by a new Azoarcus species |
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Authors: | J Harder |
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Institution: | Max-Planck-Institut für marine Mikrobiologie, Abteilung Mikrobiologie, Celsiusstra?e 1, D-28359 Bremen, Germany Tel. +49-421-2028-750; Fax +49-421-2028-580 e-mail: jharder@mpi-bremen.de, DE
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Abstract: | A bacterium, strain 22Lin, was isolated on cyclohexane-1,2-diol as sole electron donor and carbon source and nitrate as electron
acceptor. Cells are motile rods and are facultatively anaerobic. A phylogenetic comparison based on the total 16S rRNA gene
sequence allowed the assignment of the isolate to the genus Azoarcus. Cyclohexanol, cyclohexanone, cyclohexane-1,3-diol, and cyclohexane-1,3-dione, which are oxidized by a different denitrifying
strain, did not support denitrifying growth of isolate 22Lin. On the contrary, cyclohexanol (I50 = 37 μM) and cyclohexanone (I50 = 28 μM) inhibited growth on cyclohexane-1,2-diol, but not on acetate. NAD was reduced by crude extracts of strain 22Lin
in the presence of cyclohexane-1,2-dione, but not in the presence of cyclohexanone or cyclohexane-1,3-dione. The formation
of 6-oxohexanoate from cyclohexane-1,2-dione and of adipate during NAD reduction suggests that strain 22Lin possesses a carbon–carbon
hydrolase that transforms cyclohexane-1,2-dione into 6-oxohexanoate. This pathway was once observed in an aerobic pseudomonad
that was lost and could not be reisolated. Here, the application of strictly anoxic enrichment conditions enabled the reisolation
of another strain (22Lin) that uses this pathway.
Received: 3 February 1997 / Accepted: 12 May 1997 |
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Keywords: | Azoarcus Diol dehydratase Acetoin dehydrogenase Carbon carbon hydrolase Denitrification |
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