A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins |
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Authors: | Edwards R J Bentley N J Carr A M |
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Institution: | MRC Cell Mutation Unit, Sussex University, Falmer BN1 9RR, UK. |
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Abstract: | The conserved PIK-related kinase Rad3 is required for all DNA-integrity-checkpoint responses in fission yeast. Here we report a stable association between Rad3 and Rad26 in soluble protein extracts. Rad26 shows Rad3-dependent phosphorylation after DNA damage. Unlike phosphorylation of Hus1, Crb2/Rhp9, Cds1 and Chk1, phosphorylation of Rad26 does not require other known checkpoint proteins. Rad26 phosphorylation is the first biochemical marker of Rad3 function, indicating that Rad3-related checkpoint kinases may have a direct role in DNA-damage recognition. |
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