Complete sequence of the binary vector Bin 19 |
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Authors: | David A Frisch Larry W Harris-Haller Nathaniel T Yokubaitis Terry L Thomas Susan H Hardin Timothy C Hall |
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Institution: | (1) Institute of Developmental and Molecular Biology, Texas A&M University, 77843-3155 College Station, TX, USA;(2) Department of Biology, Texas A&M University, 77843-3155 College Station, TX, USA;(3) Present address: DeKalb Plant Genetics, 62 Maritime Drive, 06355 Mystic, CT, USA |
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Abstract: | Despite the widespread use of Bin 19 as a vector for plant transformation, detailed sequence information on its T-DNA region has only recently become available. We now show that the non-T-DNA region, like the T-DNA region, contains several superfluous insertions and find that some functional elements may not contain optimal sequences. Knowledge of the complete 11 777 bp sequence will aid in the construction of exceptionally efficient derivative vectors of approximately half this size. Precise knowledge of restriction sites and removal of unnecessary sequences will facilitate plasmid manipulations and plant transformation. |
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Keywords: | Bin 19 binary vector t-DNA |
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