| Abstract: | We have treated Spisula and Asterias oocytes with quercetin to determine the effects of this drug on germinal vesicle breakdown (GVBD). Quercetin (100-200 microM) reversibly inhibited GVBD when induced by excess KCl or ionophore A23187. Kinetic studies showed that quercetin blocked an early event in GVBD. Lower concentrations of quercetin (10-20 microM) blocked fertilization. However, quercetin sensitized the oocytes to initiation of GVBD by excess ions which do not normally trigger GVBD. Quercetin (100-200 microM) also blocked 1-methyladenine-induced GVBD in the starfish. In subthreshold concentrations of the hormone or in its absence, lower concentrations (20-40 microM) stimulated GVBD. The results support the hypothesis that quercetin exerts its effects on meiosis initiation through its effects on calcium sequestration. |
