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| 一种实用的碳纤电极制作方法 | |
| 作者姓名： | Cai D Qu AL Wang XM Zhou Z Xu JH Han JS |
| 作者单位： | 1. 华中理工大学生物物理与生物化学研究所,武汉,430074 2. 北京医科大学神经科学研究所 3. 中国科技大学生命科学学院 |
| 基金项目： | 国家自然科学基金!资助项目 (No 6 95710 14 3952 50 0 9,39480 0 0 8),九五科技攻关计划! (96 90 6 0 5 0 8) |
| 摘 要： | 碳纤电极记录可用于对单个神经及内分泌细胞分泌过程进行电化学检测,其中技术关键之一是碳纤电极的制作。本文所介绍的碳纤的制作方法,简化了以往的工艺过程,使成功率与一致性得到了提高,同时检测灵敏度与噪声等指标满足了实验要求。用这样制作的碳纤电极在大鼠肾上腺嗜铬细胞上研究１－甲基－４－苯基毗啶对于单胺能神经细胞递质胞吐过程的影响,发现ＭＰＰ＾＋对于胞吐没有直接的影响并且不增加高Ｋ＾＋刺激所诱发的胞吐量。从 |
| 关 键 词： | 碳纤电极 分泌 肾上腺嗜铬细胞 电化学测量 MPP |
A method for preparation of carbon fiber electrode |
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| Cai D,Qu AL,Wang XM,Zhou Z,Xu JH,Han JS.A method for preparation of carbon fiber electrode[J].Acta Physiologica Sinica,1999,0(6):692-699. | |
| Authors: | Cai D Qu A L Wang X M Zhou Z Xu J H Han J S |
| Institution: | Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan, 430074. |
| Abstract: | Carbon fiber electrode (CFE) can be used in detection of exocytosis of single neuron or endocrine cell. A simplified method for preparation of CFE, as described in the present paper, greatly ensures the coordination and success of the preparation. Such CFEs have a low noise level. Exocytosis of rat adrenal chromaffin cells was investigated in the clarification of the effect of MPP on transmitter release. It is found that exocytosis was not stimulated by MPP and the high K induced secretion was not changed by MPP . These observations suggest that the previously reported elevation of dopamine content in the surrounding histosolution of DA neuron is probably resulted from blockage of the re uptake of DA by MPP . |
| Keywords: | carbon fiber electrode neurotransmitter release adrenal chromaffin cell electrochemical detection MPP |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |