p82H identifies sequences at every human centromere |
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| Authors: | Carmen Aleixandre Dorothy A Miller Arthur R Mitchell Dorothy A Warburton Steven L Gersen Christine Disteche Orlando J Miller |
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| Institution: | (1) Department of Molecular Biology and Genetics, Wayne State University School of Medicine, 48201 Detroit, MI, USA;(2) Department of Genetics and Development, Columbia University, 10032 New York, NY, USA;(3) Morristown Memorial Hospital, 07960 Morristown, NJ, USA;(4) Department of Pathology, University of Washington, 98195 Seattle, WA, USA;(5) Dt. Ciencias Morfologicas, Faculdad Medicina, Universidad de Salamanca, 37007 Salamanca, Spain;(6) 540 East Canfield, Scott Hall Room 3216, 48201 Detroit, MI, USA |
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| Abstract: | Summary A cloned alphoid sequence, p82H, hybridizes in situ to the centromere of every human chromosome. After washing under stringent conditions, no more than 8% of the grains are located on any specific chromosome. p82H thus differs from other centromeric sequences which are reported to be chromosome specific, because it detects sequences that are conserved among the chromosomes. Two experimental approaches show that the p82H sequences are closely associated with the centromere. First, p82H remains with the relocated centromeres in an inv(19) and an inv(6) chromosome. Second, p82H hybridizes at the centromere but not to the centromeric heterochromatin of chromosomes 1, 9 and 16 that have elongated 1qh, 9qh and 16qh regions produced by short growth in 5-azacytidine. The only noncentromeric site of hybridization is at the distal end of the 9qh region. |
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