| 香菇原生质体诱变及营养缺陷型突变体筛选 |
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| 引用本文： | 杨崇林,张鉴铭.香菇原生质体诱变及营养缺陷型突变体筛选[J].云南植物研究,1992,14(2):187-192. |
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| 作者姓名： | 杨崇林 张鉴铭 |
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| 作者单位： | 中国科学院昆明植物研究所 昆明650204
(杨崇林)，中国科学院昆明植物研究所 昆明650204(张鉴铭) |
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| 基金项目： | 国家自然科学基金，云南省应用基础基金 |
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| 摘 要： |
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| 关 键 词： | 突变性 营养缺陷型 原生质体 香菇 |
INDUCTION AND SELECTION OF AUXOTROPHIC MUTANTS FROM THE PROTOPLASTS OF LENTINUS EDODES |
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| YANG Chong-Lin,ZHANG Jian-Ming.INDUCTION AND SELECTION OF AUXOTROPHIC MUTANTS FROM THE PROTOPLASTS OF LENTINUS EDODES[J].Acta Botanica Yunnanica,1992,14(2):187-192. |
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| Authors: | YANG Chong-Lin ZHANG Jian-Ming |
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| Abstract: | Protoplasts isolated from monokaryotic mycelia of Lentinus edodes were spread on CM containing 0.6 mol/1 sucrose and irradiated for 30 seconds. After colonies formed, they were transplanted on MM and those showing little growth were detected further. 7 auxotrophic mutants were obtained at last, among which 3 mutants remained stable after protoplast isolation, regeneration and mycelial subculture on CM slant for 3 times. They can be used in protoplast fusion in later studies, despite their protoplast yields were low and have to be improved. |
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| Keywords: | Lentinus edodes Protoplast Auxotrophic mutant |
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