Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon |
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Authors: | Koichiro Shimomura Hiroshi Sudo Hitoshi Saga Hiroshi Kamada |
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Affiliation: | (1) Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305 Ibaraki, Japan;(2) Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305 Ibaraki, Japan |
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Abstract: | Summary Hairy root cultures of Lithospermum erythrorhizon were established by transformation of in vitro grown shoots with Agrobacterium rhizogenes 15834. Hairy roots cultured on Murashige and Skoog solid medium did not produce any red pigments. However, the hairy roots cultured in Root Culture solid or liquid media produced a large amount of red pigments, which were released to the medium. The addition of adsorbents to the culture medium stimulated shikonin production by ca. 3-fold. Using this method an air-lift fermenter system was established, equipped with a XAD-2 column, which continuously produced ca. 5 mg/day of shikonin during a period of more than 220 days. |
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Keywords: | Lithospermum erythrorhizon Hairy root culture Shikonin Secretion Adsorbent |
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